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Enhanced immunogenicity for CD8+ T cell induction and complete protective efficacy of malaria DNA vaccination by boosting with modified vaccinia virus Ankara | Nature Medicine
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Enhanced immunogenicity for CD8+ T cell induction and complete protective efficacy of malaria DNA vaccination by boosting with modified vaccinia virus Ankara

Nature Medicine volume 4, pages 397–402 (1998)Cite this article

Abstract

Immunization with irradiated sporozoites can protect against malaria infection and intensive efforts are aimed at reproducing this effect with subunit vaccines. A particular sequence of subunit immunization with pre-erythrocytic antigens of Plasmodium berghei, consisting of single dose priming with plasmid DNA followed by a single boost with a recombinant modified vaccinia virus Ankara (MVA) expressing the same antigen, induced unprecedented complete protection against P. berghei sporozoite challenge in two strains of mice. Protection was associated with very high levels of splenic peptide-specific interferon-γ-secreting CD8+ T cells and was abrogated when the order of immunization was reversed. DNA priming followed by MVA boosting may provide a general immunization regime for induction of high levels of CD8+ T cells.

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Authors and Affiliations

  1. Molecular Immunology Group, Institute of Molecular Medicine, Nuffield Department of Medicine, University of Oxford, John Raddiffe Hospital, Oxford, 0X3 9DS, UK

    Jörg Schneider, Tomas Hanke, Carolyn M. Hannan & Adrian V.S. Hill

  2. Wellcome Trust Centre for Human Genetics, University of Oxford, Windmill Road, Headington, Oxford, 0X3 7BN, UK

    Sarah C. Gilbert & Adrian V.S. Hill

  3. Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK

    Tom J. Blanchard, Marion Becker & Geoffrey L. Smith

  4. MRC Molecular Haematology Unit, Institute of Molecular Medicine, University of Oxford, Oxford, 0X3 9DS, UK

    Kathryn J. Robson

  5. Molecular and Cellular Parasitology Group, Department of Pure and Applied Biology, Imperial College, London, UK

    Robert Sinden

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Schneider, J., Gilbert, S., Blanchard, T. et al. Enhanced immunogenicity for CD8+ T cell induction and complete protective efficacy of malaria DNA vaccination by boosting with modified vaccinia virus Ankara. Nat Med 4, 397–402 (1998). https://doi.org/10.1038/nm0498-397

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